QB Issue Resolution:

Undoing a bank statement reconciliation should be done on each transaction involve.

Here’s how:

  1. Go to Accounting from the left menu.
  2. Go to Chart of Accounts tab.
  3. Locate the account and click View register. 
  4. Change the R status to C under the check column.
  5. Repeat the changing status for every transaction.

For additional information, please refer to this article: https://quickbooks.intuit.com/community/Banking-and-bank-feeds/How-to-unreconcile-a-transaction-or-u…

Resolution for Issue 'Can I undo a bank statement reconciliation?' available: Yes (Solved).
Source: Intuit Community forum.

Can I undo a bank statement reconciliation?: this issue or error code is a known issue in Quickbooks Online (QBO) and/or Quickbooks. Support for this issue is available either by self-service or paid support options. Experts are available to resolve your Quickbooks issue to ensure minimal downtime and continue running your business. First try to resolve the issue yourself by looking for a resolution described below. If it is a complex issue or you are unable to solve the issue, you may contact us by clicking here or by using other support options.

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