QB Issue Resolution:

Check Version of updates are the same on both PC’s. Also, you cannot use the same QB section at the same time ie., Vendors entering data only from one PC. If they still don’t talk to each PC then would from #1 PC that has the master QB file go to File – Utilities – rebuild and verify data after you do a back-up and have QB closed on #2 PC then reboot all and open QB to run as in the past. Hope this works.

Resolution for Issue 'Multi-user access stopped working.' available: Yes (Solved).
Source: Intuit Community forum.

Multi-user access stopped working.: this issue or error code is a known issue in Quickbooks Online (QBO) and/or Quickbooks. Support for this issue is available either by self-service or paid support options. Experts are available to resolve your Quickbooks issue to ensure minimal downtime and continue running your business. First try to resolve the issue yourself by looking for a resolution described below. If it is a complex issue or you are unable to solve the issue, you may contact us by clicking here or by using other support options.

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